Current Research in Agriculture and Farming (CRAF)
Year : 2020, Volume : 1, Issue : 2
First page : (15) Last page : (20)
Article doi: : http://dx.doi.org/10.18782/2582-7146.103
Mamta Kamboj1*, D. S. Chauhan2 and Rupender Kumar3
1Department of Chemistry, Kurukshetra University, Kurukshetra-136119, Haryana, India
2National Seeds Corporation Ltd, Mohali, Punjab, India
3CCS Haryana Agricultural University, Hisar, Haryana 125004 India
*Corresponding Author E-mail: mamtavashu23@gmail.com
Received: 7.05.2019 | Revised: 13.06.2020 | Accepted: 20.06.2020
ABSTRACT
The seed is a significant part and the quality seed plays a urgent job in farming creation just as in national economy. In this manner, the great quality seed is essential to improve the creation and profitability. Seed have most extreme life at the hour of physiological development, from that point the seed steadily matured and decreases in feasibility and energy. Seed maturing prompts decrease in seed quality execution and stand foundation. So different preparing procedures and strategy is utilized to test the aliveness of seeds. Present technique depict the germination of seeds by means of decrease of drab tetrazolium salt reagent into hued formazan. The quantification of germination i.e. how much the seed is germinated can be test by measuring the absorbance of colored formazan using uv-visible spectrophotometer. Improved seed empowerment strategies being utilized in numerous pieces of world to decrease the germination time, synchronize germination, improve germination rate and increment seedling stand.
Keywords: Seed; Ageing; Tetrazolium Salt; Spectrophotometer.
Cite this article: Kamboj, M., Chauhan, D.S., & Kumar, R. (2020). Novel Colorimetric Method to Measure Aliveness of Seed Samples via Tetrazolium Salt as Analytical Reagent, Curr. Rese. Agri. Far. 1(1), 15-20. doi: http://dx.doi.org/10.18782/2582-7146.103