Current Research in Agriculture and Farming (CRAF)
Year : 2026, Volume 7, Issue 4
First page : 1-14
Article doi: : http://dx.doi.org/10.18782/2582-7146.344
Next Generation Sequencing (NGS): A Breakthrough Technique for Plant Virus Detection
1Research Scholar, Department of Nematology, College of Agriculture, OUAT, Bhubaneswar, Odisha 2Highly Skilled Researcher, Department of Biotechnology and Bioinformatics, ICAR- Directorate of Onion and Garlic Research, Pune, Maharashtra 3Research Scholar, Department of Botany, University of Allahabad, Uttar Pradesh 4B.Sc. Scholar, ICAR- IARI, Jharkhand 5Research Scholar, Department of Genetics and Plant Breeding, Uttar Banga Krishi Vishwavidyalaya, Pundibari, Coochbehar 6M.Sc., College of Agriculture, Central Agricultural University, Iroisemba, Imphal – 795004, Manipur *Corresponding Author E-mail: rkswadhinkumar@gmail.com Received: 2.06.2026 | Revised: 28.07.2026 | Accepted: 13.08.2026
ABSTRACT
Next Generation Sequencing (NGS) has emerged as a powerful and high-throughput technology for the rapid and comprehensive detection of plant viruses. Unlike conventional diagnostic methods that require prior knowledge of viral pathogens, NGS enables the identification of both known and novel viruses directly from infected plant tissues. By generating millions of sequence reads, NGS facilitates viral genome reconstruction, characterization of mixed infections, and detection of divergent or emerging viral strains. Its application has significantly improved plant virus surveillance, epidemiological studies, and disease management. Integration of NGS with advanced bioinformatics tools further enhances sequence analysis, pathogen identification, and phylogenetic investigation. Despite challenges related to cost, data processing, and standardization, NGS represents a transformative approach for early and accurate plant virus diagnosis and offers substantial opportunities for sustainable crop protection and biosecurity.
Keywords: Next Generation Sequencing, Plant Viruses, Virus Detection, Metagenomics, Bioinformatics
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2582-7146.344
Cite this article:
Swain, S. K., Shinde, D. D., Soni, R., Krishna, V., Das, P., & Kumar, R. (2026). Next Generation Sequencing (NGS): A Breakthrough Technique for Plant Virus Detection , Curr. Rese. Agri. Far. 7(4), 1-14. doi: http://dx.doi.org/10.18782/2582-7146.344
